Automated plate microscope for live cell, Zebrafish and Organoid imaging
The latest addition to the high-throughput automated microscope line, the CellDiscoverer 7 LSM900 Airyscan2 is installed in L3 confined space for imaging infected live cells by full field fluorescence microscopy or confocal imaging.
The Celldiscoverer 7 is a fully integrated, high-end automated live cell imaging system. Tailor the system to your applications with various incubation and detection options. A hardware focuser automatically detects and maintains focus after detecting the thickness and optical properties of the sample holder for demanding long-term imaging. Autocorr objectives correct for spherical aberrations to provide sharp contrast and high resolution. The automated microscope offers a range of built-in incubation options to create the right environment for your live cells.
A multimodal approach:
– Acquisition automation
– High sensitivity confocal imaging with GaAsP detectors and Airyscan 2
– Fast imaging thanks to the multiplex mode
– Increased resolution thanks to the AiryScan 2
– Combined mode for full-field and confocal acquisitions
Objectives (adaptive optics) :
   Zeiss Plan Apochromat 2,5x/0,12 5x/0,25 10x/0,35 (compatible with plastics and glass from 0.13 to 1.2 mm)
   Zeiss Plan Apochromat 10x/0,35 20x/0,7 40x/0,7 (fits plastics and lenses from 0.13 to 1.2 mm)
   Zeiss Plan Apochromat water immersion 25xx /1,.2 50x/1,2 100x/1,2 (fits plastics and glass from 0.13 to 0.21 mm)
BF illumination: brightfield contrast, oblique contrast, adaptive phase contrast (PGC)
Fluorescence: Fluorescence illumination unit composed of 4 LEDs divided into 2 Colibri units
Confocal Imaging LSM900 with 405, 488, 561 and 640 nm diode laser bench
Imaging modes:
1- WF mode acquisition. It uses the camera mode and allows a fast scan. It can be used as final imaging or as a fast prescan.
2- WF acquisition + GPU deconvolution (24GB): used for fast optical sectioning type acquisitions on very large fields.
3- Confocal LSM 900 acquisition. It has all the characteristics of a traditional confocal (including FRAP etc). It is mainly used for punctual applications on areas of interest determined after a camera prescan (Mode 1).
4 – Airyscan Confocal Multiplex 2 acquisition: This allows to have not only a scan speed multiplied by 2 but also a gain in sensitivity. This modality is applied either on large fields, or on regions of interest
5- Airyscan Super Resolution Multiplex 2 acquisition: this allows an increase in resolution by a factor of 1.5x and a scanning speed in SR mode multiplied by a factor of 2. This modality focuses on regions of interest
The confocal modalities can be combined with a WF acquisition to target only the color of interest in confocal or SR mode.
Control with ZEN Blue software
Translated with www.DeepL.com/Translator (free version)
The latest addition to the high-throughput automated microscope line, the CellDiscoverer 7 LSM900 Airyscan2 is installed in L3 confined space for imaging infected live cells by full field fluorescence microscopy or confocal imaging.
The Celldiscoverer 7 is a fully integrated, high-end automated live cell imaging system. Tailor the system to your applications with various incubation and detection options. A hardware focuser automatically detects and maintains focus after detecting the thickness and optical properties of the sample holder for demanding long-term imaging. Autocorr objectives correct for spherical aberrations to provide sharp contrast and high resolution. The automated microscope offers a range of built-in incubation options to create the right environment for your live cells.
A multimodal approach:
– Acquisition automation
– High sensitivity confocal imaging with GaAsP detectors and Airyscan 2
– Fast imaging thanks to the multiplex mode
– Increased resolution thanks to the AiryScan 2
– Combined mode for full-field and confocal acquisitions
Equipment :
Objectives (adaptive optics) :
   Zeiss Plan Apochromat 2,5x/0,12 5x/0,25 10x/0,35 (compatible with plastics and glass from 0.13 to 1.2 mm)
   Zeiss Plan Apochromat 10x/0,35 20x/0,7 40x/0,7 (fits plastics and lenses from 0.13 to 1.2 mm)
   Zeiss Plan Apochromat water immersion 25xx /1,.2 50x/1,2 100x/1,2 (fits plastics and glass from 0.13 to 0.21 mm)
BF illumination: brightfield contrast, oblique contrast, adaptive phase contrast (PGC)
Fluorescence: Fluorescence illumination unit composed of 4 LEDs divided into 2 Colibri units
Confocal Imaging LSM900 with 405, 488, 561 and 640 nm diode laser bench
Imaging modes:
1- WF mode acquisition. It uses the camera mode and allows a fast scan. It can be used as final imaging or as a fast prescan.
2- WF acquisition + GPU deconvolution (24GB): used for fast optical sectioning type acquisitions on very large fields.
3- Confocal LSM 900 acquisition. It has all the characteristics of a traditional confocal (including FRAP etc). It is mainly used for punctual applications on areas of interest determined after a camera prescan (Mode 1).
4 – Airyscan Confocal Multiplex 2 acquisition: This allows to have not only a scan speed multiplied by 2 but also a gain in sensitivity. This modality is applied either on large fields, or on regions of interest
5- Airyscan Super Resolution Multiplex 2 acquisition: this allows an increase in resolution by a factor of 1.5x and a scanning speed in SR mode multiplied by a factor of 2. This modality focuses on regions of interest
The confocal modalities can be combined with a WF acquisition to target only the color of interest in confocal or SR mode.
Control with ZEN Blue software
Translated with www.DeepL.com/Translator (free version)
Publications:
Chable-Bessia, C. et al., 2022. Low Selectivity Indices of Ivermectin and Macrocyclic Lactones on SARS-CoV-2 Replication In Vitro. COVID 2022, 2, 60-75. https://doi.org/10.3390/covid2010005
Laplantine et al., 2022. The FDA-approved drug Auranofin has a dual inhibitory effect on SARS-CoV-2 entry and NF-κB signaling, iScience 25 (10)
https://doi.org/10.1016/j.isci.2022.105066.
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