BSL-3 Core Facilities

6 boxes of the BSL3 facilities are dedicated to either viruses or bacterias and contain state-of-the-art equipment, including: 

• 16 Biological Safety Cabinets
• Shaked, CO2, tri-gaz incubators
• 4°C refrigerators, -20°C, -80°C and -150°C freezers
• Small and medium capacity centrifuges
• 2 ultracentrifuges

  • Optima L80 XP BioSafe (BECKMAN COULTER)

  • Optima Max XP BioSafe (BECKMAN COULTER)

• Optical microscopes 

  • AMF-4302 EVOS® digital inverted microscope (Fluorescence)

  • AMF-4300 EVOS® digital inverted microscope (Fluorescence)

  • AMF-4304 EVOS® digital inverted microscope (Fluorescence)

  • AMEX 1100 EVOS® Core XL digital inverted microscope (Bright Field & phase contrast)  

A box equipped with a NovoCyte Flow Cytometer 2100Y installed in a Biological Safety Cabinet. 

• 2 lasers (blue 488nm and yellow 561nm), which can be upgraded with a red or violet laser.
• 10 fluorescence parameters
• Absolute counting, without addition of counting beads
• Sample tube/plate holder (24 tube rack or 24-48-96 well plates)
• NovoExpress Software is enable to analyze cell cycle with Watson’s method (Cytometry 2001 ;43:55-68)

->Prices

Our BSL3 is equipped with a workstation specifically designed for automated drug screening on cell-based assays

• A Zephyr multichannel liquid handler with 96-and 384-well capabilities for performing accurately and efficiently plate preparation, replication or reformatting, serial dilutions and cell based assays.
• A Biotek plate washer.
• An Envision Multilabel reader (Perkin Elmer). This fast and effective reader can accommodate a wide range of mirror modules and filters for a variety of detection approaches based on fluorescence, luminescence and absorbance.
• A Twister II Microplate Handler with multiple instrument capability, integral plate and tip storage capacity.
• A microplate incubator LiCONiC STX44 (2 racks for a capacity of 44 microplates)

The workstation is supported by iLinkPro, a robust and easy to use control software that connects all the instruments to set up a fully automated assay system.